General Pharmacology (Practical)

List of Practicals

1. 12 experiments must be performed) To describe the contributions of renowned pharmacologists and their discoveries based on pharmacological experiments (Any 5 Noble Laureates whose research contributed to the development of Pharmacology). To study various laboratory safety precautions, hazards, personal hygiene, commonly used tools, devices and instruments in experimental pharmacology. To study common experimental animals including transgenic animals along with their applications in the pharmacological experiments in current drug discovery paradigm. To study concept of 6Rs along with the maintenance and experimentation laboratory animals as per the CCSEA guidelines. To demonstrate collection/isolation of DNA and RNA using computer simulations and audiovisual aids. To study important anaesthetics and euthanasia procedures for experimental animals. To demonstrate different routes drug administration using computer simulation and understand the significance of each route along with the maximum administrable dose. To study preparation of different types of physiological salt solutions (PSS), cell culture media and to understand the role of each ingredient used in preparation. To study the instrumentation used for isolated tissue experiments (students organ bath assembly) and recent development in recording of the responses isolated tissues. To record the dose response curve of any two agonists on suitable isolated tissue preparation using computer simulation experiment. To study the potentiating effect of physostigmine on DRC of acetyl choline through interactive computer simulation. To study antagonizing effect of d-tubocurarine on the DRCs of acetylcholine through interactive computer simulation. To determine of PD2 of given agonists using isolated tissue preparation using computer simulation experiment. To study and determine various pharmacokinetic parameters (Cmax, Tmax, t½, Vd, Clt, AUC, AUMC) from given hypothetical data. To estimate LD₅₀ using hypothetical data through computer-simulated experimentation (as per OECD 425 guideline) using software. Software based quantification of micronucleus test and chromosomal aberration test Software based prediction of teratogenicity. Use of UV-Spectrophotometer for studying drug-protein interactions. *PCI recommended software’s shall be used for performing experiments.