Basic Training in Aseptic Handling Techniques

List of Practicals

1. Gown-up / Gown-down Drill – correct sequencing of coverall, hood, mask, goggles, gloves, and sterile boots.
2. Hand-washing Verification – surgical scrub with UV lotion; inspect under UV lamp for missed spots.
3. Clean-room Entry & Flow Simulation – air-shower pass, unidirectional walking, and material air-lock transfer.
4. LAF Hood Preparation – wipe-down with sporicidal, set airflow, place sterile tools in first-air zone.
5. Smoke-pattern Test – visualize HEPA downflow with smoke to confirm
6. Aseptic Liquid Transfer – pipette or syringe transfer of TSB between vials without touching non-sterile surfaces.
7. Sterile Filtration & Filter Integrity Check – filter a buffer through 0.22 and perform bubble-point test.
8. Mini Media-Fill – fill 20 sterile vials with TSB under LAF, incubate, record turbidity for 14 days.
9. Environmental & Personnel Monitoring – deploy settle/contact plates, take glove-fingertip prints, run an air sampler.
10. Cleaning & ATP Validation – full hood wipe-down, then ATP swab pre- post-clean to verify < 200 RLU residue.