Semester VII · PCI syllabus 2026
Modern Analytical Techniques
BP704T
3 credits
Theory
Coming soon
Unit 1: 1. Nuclear Magnetic Resonance Spectroscopy:
- Principles of ¹H-NMR and ¹³C-NMR, various solvents used, chemical shift, factors affecting chemical shift, coupling constant, spin–spin coupling, relaxation, instrumentation of FT-NMR and its applications
- 2. Mass Spectrometry: Principles, fragmentation and its rules, ionization techniques – Electron impact, chemical ionization, MALDI, FAB, API, analyzers – Time of flight and quadrupole, ion trap, detectors and applications
Unit 2: 1. X-Ray Diffraction Methods:
- Origin of X-Rays, basic aspects of crystals, X-Ray crystallography, rotating crystal technique, single crystal diffraction, powder diffraction, structural elucidation and applications
- 2. Thermal Analysis: Introduction, instrumentation, factors affecting measurements, applications of TGA, DSC (types) and DTA
Unit 3: 1. UPLC and Nano LC: Principle, advantages over LC and
- applications
- 2. Principle and applications of hyphenated techniques: GC-MS, LC- MS/MS, ICP-MS
- 3. Supercritical chromatography and flash chromatography: principles and applications
Unit 4: 1. Green Analytical Chemistry:
- Types of green solvents, various computational tools used to assess the greenness and its applications in sample preparation and analytical method development
- 2. Bio-analytical Methods: Introduction to bioanalytical method development, extraction of drugs and metabolites from biological fluids – SPE, LLE, PPE, BCS classification, PK-PD interaction, microsomal assays, MTT assay, BA & BE study protocol, biosimilars
- 3. Radio Immune Assays and ELISA: Importance, various components, principle, different methods, limitations and applications of radio immunoassay and ELISA
Unit 5: 1. Microscopy-Based Analytical Techniques:
- Principle, instrumentation, and applications of optical microscopy, scanning electron microscopy and transmission electron microscopy
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