Systemic Pharmacology I (Practical)

List of Practicals

1. 12 Experiments must be performed) To demonstrate Langendorff’s heart assembly and its applications in pharmacology video demonstration/or charts and illustrations). Recording of the effects of different electrolytes, agonists and antagonists on perfused frog heart preparation using interactive computer simulation experiment. To study the effect of various drugs on blood pressure and heart rate anaesthetized interactive computer simulation experiment. Demonstration of the estimation of intraocular pressure on rabbit eye and human conventional Schiotz tonometer and non-contact tonometer. To evaluate the muscle relaxant activity of drugs on Rota-rod apparatus using computer simulation experiment. Demonstration of the effect of spasmogens and spasmolytic using rabbit interactive computer simulation experiment. Determination of PA2 value of Atropine using a suitable isolated tissue preparation plot method with the help of hypothetical data using interactive computer experiment. Determination of PA2 value of Prazosin using a suitable isolated tissue preparation plot method with the help of hypothetical data using interactive computer experiment. To study the antiallergic effects of drugs using mast cell degranulation assay computer simulated experiment. Evaluation of diuretic activity of drugs in rats using metabolic cages (simulation) of electrolytes in the urine samples/serum using flame photometer/commercially Evaluation of effects of antihistaminic drugs on the histamine-induced bronchospasm pigs using interactive simulated experiment. To study and analyse drug-drug interaction/ rational drug management of asthma of Case Study/ hypothetical data/ any clinical report. To study clinical pharmacology of the plasma volume expanders and their importance charts/open sources videos). Demonstration of evaluation of anti-inflammatory activity in paw oedema plethysmometer using simulations. Concept of Biobanking and its significance in drug screening. Isolation of Polymorphonuclear cells from blood and evaluation of endotoxin-induced stress in them using Nitrobluetetrazolium dye method by colourimetry. Determination of viability of cells (using isolated polymorphonuclear cells) by exclusion assay to determine cell viability using microscopy method. Determination of anti-inflammatory activity of durgs in vitro using RBC stabilization assay Determination of protein concentrations using Bradford reagent and plotting of of bovine serum albumin. Estimation of anticholinesterase inhibitory activity of drugs using Ellman’s reagent Determination and and plotting of standard curve for LPO, Nitric oxide and GSH. *PCI recommended software’s shall be used for performing experiments.