Pharmaceutical Biotechnology (Practical)

List of Practicals

1. Understanding Good microbiological laboratory practices while working Microbiology laboratory aseptic handling in microbiology lab: growth and isolation of microbes using streaking, spreading and subculturing techniques: microbial staining, study of various equipments such as loop, straight wire, spreader, pipette, test tube, petridish, burner etc and apparatus used in microbiology BOD incubator, laminar flow, aseptic hood, autoclave, hot air sterilizer, freezer and microscopes.
2. Handling of biological spill, decontamination procedures and hygiene handling microorganisms and disposal
3. Sterilization and evaluation of glassware and apparatus using hot air oven
4. Preparation and sterilization of solid and liquid culture medium using different
5. Determination of microbiological efficacy of disinfectant/ preservative efficacy
6. Tests for sterility of ophthalmic or parenteral marketed formulation according
7. Analysis of Biotechnological product (Protein, nucleic acid materials) by UV and FTIR spectrophotometer
8. Production of alcohol using Fermentation process
9. Practice Whole cell immobilization technique (any one)
10. Practice Enzyme immobilization technique and its kinetics
11. Isolation and estimation of DNA and RNA
12. Isolation of plasmids and expression of protein
13. Agarose gel electrophoresis of DNA/ RNA
14. SDS – polyacrylamide gel electrophoresis for proteins.
15. Microbiological assay of antibiotics.